To address these issues, we have employed in vivo two-photon microscopy to produce time-lapse images of serotonin axons in the neocortex of the adult mouse. Studies using fixed tissue have suggested that serotonin neurons might be a notable exception to this rule, but they remain inconclusive. It is widely believed that damaged axons in the adult mammalian brain have little capacity to regenerate, thereby impeding functional recovery after injury. To give a flavor of this work, here's one project that deals with recovery of function after brain injury: In this context, experience can mean anything from exercise to injury to motor learning to the natural ovarian cycle. At present, most work in the lab uses in vivo 2-photon microscopy to measure the micro-structural and functional changes that occur in the rodent brain as a result of experience.
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